Immunological Data Discovery Index
Advanced Search
  1. << Go Back
Title: A deeper analysis of the epitope/paratope of PLY-5, a mouse monoclonal antibody which recognises the conserved undecapeptide tryptophan-rich loop (ECTGLAWEWWR) of bacterial cholesterol-dependent cytolysins.
identifier: 1968800
description:
epitope description:WEWWR
antigen name:Pneumolysin
host organism:Mus musculus BALB/c
antibody name:PLY-5
aggregation:
instance of dataset
availability:
available
primaryPublications: 23159621
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1025473
landingPage: http://www.iedb.org/assay/1968800
type:
Literature
publicationVenue:
Biochem Biophys Res Commun
dates:
2013
study type: b cell assays
subject species: Streptococcus pneumoniae
fullName:
Pedro Gonzá
lez-Mené
ndez
Marcos Garcí
a-Ocañ
a
Juan R de los Toyos
method:
biological activity
name:
A deeper analysis of the epitope/paratope of PLY-5, a mouse monoclonal antibody which recognises the conserved undecapeptide tryptophan-rich loop (ECTGLAWEWWR) of bacterial cholesterol-dependent cytolysins.
description:
The epitope was deduced.
Several shortenings and alanine- and conservative-variant peptides from the originally determined epitopic region ECTGLAWEWWR from PLY protein, conserved in many bacterial cholesterol-dependent cytolysins (CDCs), were tested as inhibitors of mAb PLY-5 neutralising activity in PLY-mediated haemolysis. The minimal epitope was deduced to be WEWWR, with residues W3 and R5 essential for recognition.

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.

ImmuneData is supported by the NIAID, Grant HHSN272201700019C. Privacy

ImmuneData is developed by TechWave International, Inc. and UT Health Science Center at Houston