Reverse engineering the antigenic architecture of the haemagglutinin from influenza H5N1 clade 1 and 2.2 viruses with fine epitope mapping using monoclonal antibodies.

Title:

Reverse engineering the antigenic architecture of the haemagglutinin from influenza H5N1 clade 1 and 2.2 viruses with fine epitope mapping using monoclonal antibodies.

Dataset

identifier:	1968805
description:	epitope description:D140, S145, N171, A172, R205, K228, N268 antigen name:Hemagglutinin host organism:Mus musculus BALB/c antibody name:4c11
aggregation:	instance of dataset
availability:	available
primaryPublications:	23127859

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1025463
landingPage:	http://www.iedb.org/assay/1968805

Publication

type:	Literature
publicationVenue:	Mol Immunol
dates:	2013

Dimension

study type:	b cell assays
subject species:	Influenza A virus (A/bar-headed goose/Qinghai/1A/2005(H5N1))

Person

fullName:	Steve Rockman Sarina Camuglia Kirsten Vandenberg Chi Ong Mark A Baker Roger L Nation Jian Li Tony Velkov

Data Type

method:	flow cytometry

Study

name:	Reverse engineering the antigenic architecture of the haemagglutinin from influenza H5N1 clade 1 and 2.2 viruses with fine epitope mapping using monoclonal antibodies.
description:	Cells transfected with the WT HA protein were reactive with the mAb. Epitope residues were determined by restoration of reactivity by mutation of the unreactive clade 1 HA protein from A/Vietnam/1194/04 to match the residues in A/bar-headed goose/Qinghai/1A/2005 HA protein.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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