Probing structural variability at the N terminus of the TSH receptor with a murine monoclonal antibody that distinguishes between two receptor conformational forms.

Title:

Probing structural variability at the N terminus of the TSH receptor with a murine monoclonal antibody that distinguishes between two receptor conformational forms.

Dataset

identifier:	1970447
description:	epitope description:ECHQEEDFRVTC antigen name:Thyrotropin receptor host organism:Mus musculus BALB/c antibody name:3BD10, 3E5
aggregation:	instance of dataset
availability:	available
primaryPublications:	23183178

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1025355
landingPage:	http://www.iedb.org/assay/1970447

Publication

type:	Literature
publicationVenue:	Endocrinology
dates:	2013

Dimension

study type:	b cell assays
subject species:	Homo sapiens

Person

fullName:	Sepehr Hamidi Chun-Rong Chen Ramachandran Murali Sandra M McLachlan Basil Rapoport

Data Type

method:	flow cytometry

Study

name:	Probing structural variability at the N terminus of the TSH receptor with a murine monoclonal antibody that distinguishes between two receptor conformational forms.
description:	The epitope was deduced. The epitope-specific mAbs reacted by flow cytometry with CHO cells stably expressing the TSHR ECD tethered to the plasma membrane by a GPI anchor. For mAb 3BD10 the estimated Kd based on the fluorescence with increasing concentrations of mAb was calculated at 1.2 nM. The region CSSPPC, corresponding to loop 1, was found to affect binding to the protein by mutation to residues CPEPCD as found on the FSH receptor, but not when individual residues were deleted, suggesting the loop 1 conformation affects recognition of the loop 2-specific mAbs.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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