Probing structural variability at the N terminus of the TSH receptor with a murine monoclonal antibody that distinguishes between two receptor conformational forms.

Title:

Probing structural variability at the N terminus of the TSH receptor with a murine monoclonal antibody that distinguishes between two receptor conformational forms.

Dataset

identifier:	1970426
description:	epitope description:ECHQEEDFRVTC antigen name:Thyrotropin receptor host organism:Mus musculus BALB/c antibody name:3BD10, 3E5
aggregation:	instance of dataset
availability:	available
primaryPublications:	23183178

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1025355
landingPage:	http://www.iedb.org/assay/1970426

Publication

type:	Literature
publicationVenue:	Endocrinology
dates:	2013

Dimension

study type:	b cell assays
subject species:	Homo sapiens

Person

fullName:	Sepehr Hamidi Chun-Rong Chen Ramachandran Murali Sandra M McLachlan Basil Rapoport

Data Type

method:	ELISA

Study

name:	Probing structural variability at the N terminus of the TSH receptor with a murine monoclonal antibody that distinguishes between two receptor conformational forms.
description:	The epitope was deduced. The mAbs bound two overlapping peptides (MGCSSPPCECHQEEDFRVTCK and ECHQEEDFRVTCKDIQRIPS) that share the epitope. Alanine substitutions of TSHR loop2 residues E34, E35, and D36 severely diminished binding of mAb 3BD10 and a lower effect was found for substitution of residues F37 and V39. For 3E5 the substitution of residues E35, D36 and F37 had a strong effect on recognition and lower effect was seen for substitution of E34 and V39. The region CSSPPC, corresponding to loop 1, was found not to affect binding to the peptides in ELISA by mutation analysis.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.