Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1977962 |
| description: |
epitope description:K585, F586, K587, I588, V589, K590, E591, I592, R603, E607, E640, K641, D642, P644, G665, L667, K668, L669, N670, W671
antigen name:Genome polyprotein
host organism:Mus musculus BALB/c
antibody name:4E11
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
23569282 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1025731 |
| landingPage: |
http://www.iedb.org/assay/1977962 |
| type: |
Literature
|
| publicationVenue: |
Proc Natl Acad Sci U S A
|
| dates: |
2013
|
| study type: | b cell assays |
| subject species: | Dengue virus 2 Jamaica/1409/1983 |
| fullName: |
Kannan Tharakaraman
Luke N Robinson
Andrew Hatas
Yi-Ling Chen
Liu Siyue
S Raguram
V Sasisekharan
Gerald N Wogan
Ram Sasisekharan
|
| method: |
surface plasmon resonance (SPR)
|
| name: |
Redesign of a cross-reactive antibody to dengue virus with broad-spectrum activity and increased in vivo potency.
|
| description: |
The epitope was determined in the cited reference: Cockburn et al. (2012) Structure 20(2): 303-314 [PMID: 22285214].
The affinity of epitope-specific mAb 4E11 for binding the extracellular domain III of DENV-2 was determined by surface plasmon resonance. It was also determined by competition ELISA (KD = 5.2 nM). The mAb 4E5A, engineered for improved affinity, showed a 21-fold increase in affinity for EDIII of DV2 by SPR and 9-fold increase by competition ELISA.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
