Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1977964 |
| description: |
epitope description:K305, F306, S307, I308, D309, K310, E311, M312, K323, K325, E327, T361, N362, S363, V364, N366, L387, T388, L389, H390
antigen name:Genome polyprotein
host organism:Mus musculus BALB/c
antibody name:4E11
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
23569282 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1025731 |
| landingPage: |
http://www.iedb.org/assay/1977964 |
| type: |
Literature
|
| publicationVenue: |
Proc Natl Acad Sci U S A
|
| dates: |
2013
|
| study type: | b cell assays |
| subject species: | Dengue virus 4 Burma/63632/1976 |
| fullName: |
Kannan Tharakaraman
Luke N Robinson
Andrew Hatas
Yi-Ling Chen
Liu Siyue
S Raguram
V Sasisekharan
Gerald N Wogan
Ram Sasisekharan
|
| method: |
ELISA
|
| name: |
Redesign of a cross-reactive antibody to dengue virus with broad-spectrum activity and increased in vivo potency.
|
| description: |
The epitope was determined in the cited reference: Cockburn et al. (2012) Structure 20(2): 303-314 [PMID: 22285214].
The affinity of epitope-specific mAb 4E11 for binding the extracellular domain III of DENV-4 was determined by competition ELISA. Binding was not detected by surface plasmon resonance. The mAb 4E5A, engineered for improved affinity, showed a 447-fold increase in affinity for EDIII of DV4.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
