Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1976273 |
| description: |
epitope description:T455, R456, D457, G458, G459, S461, N462, E466, I467, R469, G472, G473, D474, R476, D477, V275, D279, N280, A281, K282, S365, G366, G367, D368
antigen name:Envelope glycoprotein gp160
host organism:Homo sapiens
antibody name:GL-VRC01
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
23539181 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1025720 |
| landingPage: |
http://www.iedb.org/assay/1976273 |
| type: |
Literature
|
| publicationVenue: |
Science
|
| dates: |
2013
|
| study type: | b cell assays |
| subject species: | Human immunodeficiency virus 1 |
| fullName: |
Joseph Jardine
Jean-Philippe Julien
Sergey Menis
Takayuki Ota
Oleksandr Kalyuzhniy
Andrew McGuire
Devin Sok
Po-Ssu Huang
Skye MacPherson
Meaghan Jones
Travis Nieusma
John Mathison
David Baker
Andrew B Ward
Dennis R Burton
Leonidas Stamatatos
David Nemazee
Ian A Wilson
William R Schief
|
| method: |
surface plasmon resonance (SPR)
|
| name: |
Rational HIV immunogen design to target specific germline B cell receptors.
|
| description: |
Contact residues D79, R81, S86, and F144 in the eOD-GT6 construct are mismatched to N276, T278, T283, I371, respectively, in the natural sequence in the Genbank record for HIV HXB2 gp120 and contact residues V30, D33, T35 have no counterpart.
The affinity of the epitope-specific antibody GL-VRC01 for the HIV gp120 outer domain construct eOD-GT6 was determined by surface plasmon resonance. The affinity of the mature antibody VRC01 for eOD-GT6 was KD = 2 nM. The eOD-GT6 construct was based on an engineered outer domain lacking the inner domain, V1, V2, V3 regions and ß20/21 with 8 additional mutations introduced, including removal of glycosylation sites. Six of these mutations improved the affinity. The construct contained 10 mutations relative to HIV HxB2 gp120.
|
| name: |
iedb
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| homePage: |
http://www.iedb.org |
