Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1989486 |
| description: |
epitope description:K346, D347, L349, H370, L372, P373, V374, R377, D379, F381, T382, Q408, H433, S442
antigen name:Epidermal growth factor receptor
host organism:Lama glama
antibody name:7D12
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
23791944 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1026704 |
| landingPage: |
http://www.iedb.org/assay/1989486 |
| type: |
Literature
|
| publicationVenue: |
Structure
|
| dates: |
2013
|
| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Karl R Schmitz
Atrish Bagchi
Rob C Roovers
Paul M P van Bergen en Henegouwen
Kathryn M Ferguson
|
| method: |
surface plasmon resonance (SPR)
|
| name: |
Structural evaluation of EGFR inhibition mechanisms for nanobodies/VHH domains.
|
| description: |
The epitope residues were calculated from [PDB: 4KRL] (pH 6.0) as the antigen residues at 4Å
atomic distance from the antibody and are the following: L325, H346, L348, P349, V350, R353, D355, F357, T358, Q384, H409, S418. From [PDB: 4KRM] (pH 3.5), the epitope residues are: K322, D323, L325, L348, P349, V350, R353, D355, F357, T358, Q384, S418. Here, the combined epitope is provided.
The affinity of epitope-specific nanobody 7D12 for domain 3 of the EGF Receptor extracellular region was determined by surface plasmon resonance. Nanobody 7D12 bound with higher affinity to domain 3 than to the entire EGFR extracellular region (KD = 219 nM).
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
