Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1026656 |
| landingPage: |
http://www.iedb.org/assay/1990304 |
| type: |
Literature
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| publicationVenue: |
Nature
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| dates: |
2013
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| study type: | b cell assays |
| subject species: | Mus musculus |
| fullName: |
Tiziana Sonati
Regina R Reimann
Jeppe Falsig
Pravas Kumar Baral
Tracy O'Connor
Simone Hornemann
Sine Yaganoglu
Bei Li
Uli S Herrmann
Barbara Wieland
Mridula Swayampakula
Muhammad Hafizur Rahman
Dipankar Das
Nat Kav
Roland Riek
Pawel P Liberski
Michael N G James
Adriano Aguzzi
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| method: |
biological activity
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| name: |
The toxicity of antiprion antibodies is mediated by the flexible tail of the prion protein.
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| description: |
The epitope-specific antibody POM8 and POM9 induced loss of cerebellar granule cells in PrPC -overexpressing cerebellar organotypic cultured slices (COCS). Neurotoxicity required the expression of Prp, as Prnp(o/o) COCS were not affected. Monovalent (Fab or scFv) or divalent antibodies (IgG or Fab 2) was neurotoxic indicating that neurotoxicity did not require crosslinking or antibody effector function or complement. Neurotoxicity did require the flexible tail domain of PrP, as COCS expressing a PrP deletion mutant lacking residues 32-93 were resistant to POM neurotoxicity.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
