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identifier: 2004751
description:
epitope description:N149, Y151, I152, K162, N164, K166, D180, H181, Y182, N198, H199, Y200, G228
antigen name:Green fluorescent protein
host organism:Camelus dromedarius
antibody name:Minimizer
aggregation:
instance of dataset
availability:
available
primaryPublications: 20010839
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1026804
landingPage: http://www.iedb.org/assay/2004751
type:
Literature
publicationVenue:
Nat Struct Mol Biol
dates:
2010
study type: b cell assays
subject species: Aequorea victoria
fullName:
Axel Kirchhofer
Jonas Helma
Katrin Schmidthals
Carina Frauer
Sheng Cui
Annette Karcher
Mireille Pellis
Serge Muyldermans
Corella S Casas-Delucchi
M Cristina Cardoso
Heinrich Leonhardt
Karl-Peter Hopfner
Ulrich Rothbauer
method:
surface plasmon resonance (SPR)
name:
Modulation of protein properties in living cells using nanobodies.
description:
The epitope residues were calculated from [PDB: 3G9A] as the antigen residues at 4Å
atomic distance from the antibody. Additional contact residue T153 is mismatched to M153 and A163 is mismatched to Val163 in the sequence found in Genbank GI: 1169893.
The affinity of epitope-specific Minimizer nanobody (GBP4) for binding to GFP was determined with an Attana 100C Quartz Crystal Microbalance.

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