| identifier: | 2004751 |
| description: |
epitope description:N149, Y151, I152, K162, N164, K166, D180, H181, Y182, N198, H199, Y200, G228
antigen name:Green fluorescent protein
host organism:Camelus dromedarius
antibody name:Minimizer
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
20010839 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1026804 |
| landingPage: |
http://www.iedb.org/assay/2004751 |
| type: |
Literature
|
| publicationVenue: |
Nat Struct Mol Biol
|
| dates: |
2010
|
| study type: | b cell assays |
| subject species: | Aequorea victoria |
| fullName: |
Axel Kirchhofer
Jonas Helma
Katrin Schmidthals
Carina Frauer
Sheng Cui
Annette Karcher
Mireille Pellis
Serge Muyldermans
Corella S Casas-Delucchi
M Cristina Cardoso
Heinrich Leonhardt
Karl-Peter Hopfner
Ulrich Rothbauer
|
| method: |
surface plasmon resonance (SPR)
|
| name: |
Modulation of protein properties in living cells using nanobodies.
|
| description: |
The epitope residues were calculated from [PDB: 3G9A] as the antigen residues at 4Å
atomic distance from the antibody. Additional contact residue T153 is mismatched to M153 and A163 is mismatched to Val163 in the sequence found in Genbank GI: 1169893.
The affinity of epitope-specific Minimizer nanobody (GBP4) for binding to GFP was determined with an Attana 100C Quartz Crystal Microbalance.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |