Kinetic analysis of sequence-specific recognition of ssDNA by an autoantibody. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Kinetic analysis of sequence-specific recognition of ssDNA by an autoantibody.

Dataset

identifier:	2006191
description:	epitope description:5'-d[CGAGCTGCCTTCAGTCG]-3' host organism:Mus musculus MRL antibody name:11F8
aggregation:	instance of dataset
availability:	available
primaryPublications:	12680766

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1016363
landingPage:	http://www.iedb.org/assay/2006191

Publication

type:	Literature
publicationVenue:	Biochemistry
dates:	2003

Dimension

study type:	b cell assays
subject species:

Person

fullName:	Jennifer A Beckingham Joanne Cleary Melissa Bobeck Gary D Glick

Data Type

method:	quenching

Study

name:	Kinetic analysis of sequence-specific recognition of ssDNA by an autoantibody.
description:	The epitope- specific mAb scFv as well as the whole antibody bound the epitope. Mutation of the antibody revealed that a group of basic residues (K62, K64, R24, K52) within the context of the binding mechanism affected binding to the epitope.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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