Thermodynamic signatures of the antigen binding site of mAb 447-52D targeting the third variable region of HIV-1 gp120.

Title:

Thermodynamic signatures of the antigen binding site of mAb 447-52D targeting the third variable region of HIV-1 gp120.

identifier:	2007042
description:	epitope description:KRIHIGPGRAFYTT antigen name:Envelope glycoprotein gp160 host organism:Homo sapiens antibody name:447-52D
aggregation:	instance of dataset
availability:	available
primaryPublications:	23944979

study type:	b cell assays
subject species:	HIV-1 M:B_MN

fullName:	April Killikelly Hui-Tang Zhang Brett Spurrier Constance Williams Miroslaw K Gorny Susan Zolla-Pazner Xiang-Peng Kong

method:	calorimetry

name:	Thermodynamic signatures of the antigen binding site of mAb 447-52D targeting the third variable region of HIV-1 gp120.
description:	The affinity of Fv 447-52D for the linear V3 MN epitope was determined by isothermal calorimetry. The ka = 0.049 1/M. Mutation of antibody heavy chain residues, Y100T, W133A, or D95A or light chain residues, W91A or W96A, reduced the affinity. Mutation of position R9Q in the epitope reduced the affinity 230-fold, whereas mutation H4R resulted in a 2.3-decrease in binding affinity.

name:	iedb
homePage:	http://www.iedb.org

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