Ligand recognition by anti-DNA autoantibodies. Affinity, specificity, and mode of binding. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Ligand recognition by anti-DNA autoantibodies. Affinity, specificity, and mode of binding.

Dataset

identifier:	2007458
description:	epitope description:dT5 host organism:Mus musculus MLR/lpr antibody name:9F11
aggregation:	instance of dataset
availability:	available
primaryPublications:	8634294

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1006970
landingPage:	http://www.iedb.org/assay/2007458

Publication

type:	Literature
publicationVenue:	Biochemistry
dates:	1996

Dimension

study type:	b cell assays
subject species:

Person

fullName:	P C Swanson C Ackroyd G D Glick

Data Type

method:	radio immuno assay (RIA)

Study

name:	Ligand recognition by anti-DNA autoantibodies. Affinity, specificity, and mode of binding.
description:	The epitope -specific mAb bound the epitope with the highest affinity, as shown by a gel shift assay. MAbs 15D8, 9F11, 15B10 are IgG2b, 8D8 is IgG2a, and 11F8 is IgG3. For the other mAbs, the KD for 11F8 was 940 nM, for 15D8 was 590 nM, for 15B10 was 230 nM, for 8D8 was 5700 nM. The epitope was the minimal DNA oligomer bound by the mAbs as shown by gel shift assay.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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