Multidonor analysis reveals structural elements, genetic determinants, and maturation pathway for HIV-1 neutralization by VRC01-class antibodies.

Title:

Multidonor analysis reveals structural elements, genetic determinants, and maturation pathway for HIV-1 neutralization by VRC01-class antibodies.

identifier:	2007331
description:	epitope description:N278, T280, N282, A283, N355, K357, S365, G366, G367, D368, I371, Q426, T449, R450, D451, G452, G453, N455, E460, G467 antigen name:Envelope glycoprotein gp160 host organism:Homo sapiens antibody name:VRC-CH31
aggregation:	instance of dataset
availability:	available
primaryPublications:	23911655

study type:	b cell assays
subject species:	Human immunodeficiency virus 1

fullName:	Tongqing Zhou Jiang Zhu Xueling Wu Stephanie Moquin Baoshan Zhang Priyamvada Acharya Ivelin S Georgiev Han R Altae-Tran Gwo-Yu Chuang M Gordon Joyce Young Do Kwon Nancy S Longo Mark K Louder Timothy Luongo Krisha McKee Chaim A Schramm Jeff Skinner Yongping Yang Zhongjia Yang Zhenhai Zhang Anqi Zheng Mattia Bonsignori Barton F Haynes Johannes F Scheid Michel C Nussenzweig Melissa Simek Dennis R Burton Wayne C Koff NISC Comparative Sequencing Program James C Mullikin Mark Connors Lawrence Shapiro Gary J Nabel John R Mascola Peter D Kwong

method:	x-ray crystallography

name:	Multidonor analysis reveals structural elements, genetic determinants, and maturation pathway for HIV-1 neutralization by VRC01-class antibodies.
description:	The epitope residues were calculated from [PDB: 4LSQ] as the antigen residues at 4Å atomic distance from the antibody. Two additional contact residue D279 and E460 in the PDB structure were mismatched to residues N281 and A454, respectively, in the GenBank sequence. The epitope of broadly and potently neutralizing antibody VRC-CH31 Fab on HIV-1 gp120 core from clade A/E 93TH057 (with loop D and Loop V5 replaced from clade A strain 3415V1) was determined from the crystal structure of the complex. The HIV-1 gp120 core also contains truncations in the variable V1/V2 and V3 loops as well as the N- and C-termini.

name:	iedb
homePage:	http://www.iedb.org

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