A unique and conserved neutralization epitope in H5N1 influenza viruses identified by an antibody against the A/Goose/Guangdong/1/96 hemagglutinin.

Title:

A unique and conserved neutralization epitope in H5N1 influenza viruses identified by an antibody against the A/Goose/Guangdong/1/96 hemagglutinin.

Dataset

identifier:	2008521
description:	epitope description:D59, D61, V63, K64, R69, E85, N88, P90, E91, H126, E128, E286, Y287, N289, N291 antigen name:Hemagglutinin host organism:Mus musculus antibody name:H5M9
aggregation:	instance of dataset
availability:	available
primaryPublications:	24049169

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1026859
landingPage:	http://www.iedb.org/assay/2008521

Publication

type:	Literature
publicationVenue:	J Virol
dates:	2013

Dimension

study type:	b cell assays
subject species:	Influenza A virus (A/Viet Nam/1203/2004(H5N1))

Person

fullName:	Xueyong Zhu Yong-Hui Guo Tao Jiang Ya-Di Wang Kwok-Hung Chan Xiao-Feng Li Wenli Yu Ryan McBride James C Paulson Kwok-Yung Yuen Cheng-Feng Qin Xiao-Yan Che Ian A Wilson

Data Type

method:	bio-layer interferometry assay

Study

name:	A unique and conserved neutralization epitope in H5N1 influenza viruses identified by an antibody against the A/Goose/Guangdong/1/96 hemagglutinin.
description:	The affinity of epitope-specific H5M9 IgG for binding to H5 HA of Influenza A virus (A/Hong Kong/156/97) was determined by bio-layer interferometry using an Octet Red instrument. For the Fab', KD = 0.9 nM. Binding of H5M9 Fab' protected the HA from degradation by trypsin at pH 4.9, suggesting that antibody binding inhibits the pH-induced conformational changes in HA needed for virus function.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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