A nanobody binding to non-amyloidogenic regions of the protein human lysozyme enhances partial unfolding but inhibits amyloid fibril formation.

Title:

A nanobody binding to non-amyloidogenic regions of the protein human lysozyme enhances partial unfolding but inhibits amyloid fibril formation.

identifier:	2008232
description:	epitope description:R28, K31, R32, G34, D36, G37, G40, I41, S42, A44, N45, V139, R140, V148 antigen name:Lysozyme C host organism:Camelus dromedarius antibody name:cAb-HuL5
aggregation:	instance of dataset
availability:	available
primaryPublications:	23919586

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Erwin De Genst Pak-Ho Chan Els Pardon Shang-Te D Hsu Janet R Kumita John Christodoulou Linda Menzer Dimitri Y Chirgadze Carol V Robinson Serge Muyldermans André Matagne Lode Wyns Christopher M Dobson Mireille Dumoulin

method:	surface plasmon resonance (SPR)

name:	A nanobody binding to non-amyloidogenic regions of the protein human lysozyme enhances partial unfolding but inhibits amyloid fibril formation.
description:	The affinity of epitope-specific nanobody cAb-HuL5 for binding to wild type human lysozyme was determined by surface plasmon resonance. Nanobody cAb-HuL5 bound with similar affinity to amyloidogenic lysozyme variants with substitution D67H (KD = 460 nM) or I56T (KD = 410 nM).

name:	iedb
homePage:	http://www.iedb.org

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