A nanobody binding to non-amyloidogenic regions of the protein human lysozyme enhances partial unfolding but inhibits amyloid fibril formation.

Title:

A nanobody binding to non-amyloidogenic regions of the protein human lysozyme enhances partial unfolding but inhibits amyloid fibril formation.

identifier:	2008235
description:	epitope description:R28, K31, R32, G34, D36, G37, G40, I41, S42, A44, N45, V139, R140, V148 antigen name:Lysozyme C host organism:Camelus dromedarius antibody name:cAb-HuL5
aggregation:	instance of dataset
availability:	available
primaryPublications:	23919586

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Erwin De Genst Pak-Ho Chan Els Pardon Shang-Te D Hsu Janet R Kumita John Christodoulou Linda Menzer Dimitri Y Chirgadze Carol V Robinson Serge Muyldermans André Matagne Lode Wyns Christopher M Dobson Mireille Dumoulin

method:	nuclear magnetic resonance (NMR)

name:	A nanobody binding to non-amyloidogenic regions of the protein human lysozyme enhances partial unfolding but inhibits amyloid fibril formation.
description:	The binding of epitope-specific nanobody cAb-HuL5 to wild type human lysozyme was studied by chemical shift perturbations in NMR spectra. Thirty-five residues, including contact residues showed significant chemical shift perturbations, indicating they were affected by cAb-HuL5 binding. Nanobody cAb-HuL5 bound to the amyloidogenic lysozyme variant with substitution I56T in a similar manner.

name:	iedb
homePage:	http://www.iedb.org

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