Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 2007890 |
| description: |
epitope description:A: K243, D246, P248, L249, N250, P380, Q381, Y382, H383; B: I231, H234, E235
antigen name:H(+)/Cl(-) exchange transporter ClcA
host organism:Mus musculus
antibody name:anti-CLC Fab
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| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
24036509 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1026856 |
| landingPage: |
http://www.iedb.org/assay/2007890 |
| type: |
Literature
|
| publicationVenue: |
Nat Chem Biol
|
| dates: |
2013
|
| study type: | b cell assays |
| subject species: | Escherichia coli K-12 |
| fullName: |
Hyun-Ho Lim
Randy B Stockbridge
Christopher Miller
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| method: |
x-ray crystallography
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| name: |
Fluoride-dependent interruption of the transport cycle of a CLC Cl-/H+ antiporter.
|
| description: |
The epitope residues were calculated from [PDB: 4KJP, 4KJQ, 4KJW, 4KK5, 4KK6, 4KKL, 4KK9, 4KKB, 4KKC, 4LOU AND 4KK8] as the antigen residues at 4Å
atomic distance from the antibody. [PDB: 4JKP and 4KKL] lack contact residue K243.
The epitope of the anti-CLC Fab on CLC-ec1, a bacterial Cl-/H+ antiporter, was determined from the crystal structure of the complex, solved by molecular replacement. The complex was equilibrated with 100 mM fluoride and 20 mM bromide. The CLC-ec1 deltaNC construct is truncated at the N and C termini. The structure contains two Fab molecules each bound to the two subunits of the homodimeric channel.
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
