Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 2012337 |
| description: |
epitope description:W69, G71, H72, F76, R111, N114, P116, F118, P119, T120, R122, S132, G133, V135, P137, V138, A139, R149
antigen name:Sulfhydryl oxidase 1
host organism:Mus musculus BALB/c
antibody name:MAb492.1
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
23867277 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1026931 |
| landingPage: |
http://www.iedb.org/assay/2012337 |
| type: |
Literature
|
| publicationVenue: |
J Mol Biol
|
| dates: |
2013
|
| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Iris Grossman
Assaf Alon
Tal Ilani
Deborah Fass
|
| method: |
biological activity
|
| name: |
An inhibitory antibody blocks the first step in the dithiol/disulfide relay mechanism of the enzyme QSOX1.
|
| description: |
The epitope-specific MAb492.1 inhibited the sulfhtdryl oxidase activity ofQSOX1 secreted from confluent fibroblasts. The IC50 = 43 nM in an assay for inhibition of RNase oxidation. The apparent inhibitory constant was determined to be Ki = 0.9 nM in an oxygen consumption assay. In a physiological assay, MAb492.1 inhibited epithelial adherence to fibroblasts and inhibited tumor cell migration on fibroblast monolayers. The Fab492.1 and scFv492.1 fragments also inhibited RNase oxidation with IC50 = 100 nM and IC50 = 250 nM, respectively.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
