Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 2012367 |
| description: |
epitope description:T68, R131, A134, I135, T136, P138, F139, A226, Q229, L230, K267, E268, A271, T274, L275, I278, Y326, R328, S329, P330
antigen name:Beta-2 adrenergic receptor
host organism:Lama glama
antibody name:Nb6B9
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
24056936 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1026932 |
| landingPage: |
http://www.iedb.org/assay/2012367 |
| type: |
Literature
|
| publicationVenue: |
Nature
|
| dates: |
2013
|
| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Aaron M Ring
Aashish Manglik
Andrew C Kruse
Michael D Enos
William I Weis
K Christopher Garcia
Brian K Kobilka
|
| method: |
biological activity
|
| name: |
Adrenaline-activated structure of beta2-adrenoceptor stabilized by an engineered nanobody.
|
| description: |
The epitope residues are calculated from the PDB structures [PDB: 4LDE, 4LDL and 4LDO] as the antigen residues at 4 Å
distance from the antibody. The epitope for PDB 4LDO does not include contact residues T1068 (T68) and A1134 (A134) and has an additional contact residue A1265, which is mismatched to C265 in the Genbank sequence.
The binding of epitope-specific nanobody Nb6B9 to ß2AR reconstituted into high-density lipoprotein particles enhanced the binding of adrenaline to ß2AR, but did not affect the binding of dihydroalprenolol.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
