Development of an ELISA for the quantification of the C-terminal decapeptide prothymosin alpha(100-109) in sera of mice infected with bacteria.

Title:

Development of an ELISA for the quantification of the C-terminal decapeptide prothymosin alpha(100-109) in sera of mice infected with bacteria.

identifier:	2014414
description:	epitope description:TKKQKTDEDD antigen name:Prothymosin alpha (UniProt:P06454) host organism:Oryctolagus cuniculus New Zealand White
aggregation:	instance of dataset
availability:	available
primaryPublications:	23831611

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Pinelopi Samara Hubert Kalbacher Kyriaki Ioannou Dorel L Radu Evangelia Livaniou Vasilis J Promponas Wolfgang Voelter Ourania Tsitsilonis

method:	antigen inhibition

name:	Development of an ELISA for the quantification of the C-terminal decapeptide prothymosin alpha(100-109) in sera of mice infected with bacteria.
description:	The free epitope inhibited binding of the epitope-specific antibodies to the biotinylated epitope. The epitope-specific antibodies also bound the biotinylated and free epitope in direct binding ELISA. The epitope-purified antibodies bound the epitope stronger then IgG purified antibodies or serum. Preincubation of proTalpha (100-109) with the antibodies increased the sensitivity of the assay.

name:	iedb
homePage:	http://www.iedb.org

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