Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1027107 |
| landingPage: |
http://www.iedb.org/assay/2021875 |
| type: |
Literature
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| publicationVenue: |
Carbohydr Res
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| dates: |
2014
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| study type: | b cell assays |
| subject species: | Trichinella spiralis |
| fullName: |
Lina Cui
Chang-Chun Ling
Joanna Sadowska
David R Bundle
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| method: |
nuclear magnetic resonance (NMR)
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| name: |
Synthesis of modified Trichinella spiralis disaccharide epitopes and a comparison of their recognition by chemical mapping and saturation transfer difference NMR.
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| description: |
Saturation transfer difference (STD) NMR was performed to evaluate the activity changes associated with monodeoxy and mono-O-methylation of disaccharides. The results established that polar contacts involved the tyvelose and GalNAc C-4 hydroxyl groups, with tyvelose C2 and GalNAc C4 and C6 hydroxyl group sitting close to or at the periphery of the binding site. The acetamido group of the GalNAc moiety is not crucial to binding, as its replacement by a hydroxyl group has only a subtle effect on activity. It should also be noted that a disaccharide in which a GlcNAc residue replaces GalNAc is inactive indicating steric constraints at the C4 position of GalNAc.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
