Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | Structural characterization of the main immunogenic region of the Torpedo acetylcholine receptor. |
| identifier: | 2022315 |
| description: |
epitope description:E26, H27, T29, R30, L31, V32, A33, N34, N92, P93, A94, Y96, I99
antigen name:Acetylcholine receptor subunit alpha
host organism:Rattus norvegicus Lewis
antibody name:mAb 132A
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
24333757 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1027151 |
| landingPage: |
http://www.iedb.org/assay/2022315 |
| type: |
Literature
|
| publicationVenue: |
Mol Immunol
|
| dates: |
2014
|
| study type: | b cell assays |
| subject species: | Tetronarce californica |
| fullName: |
Stuart W Morell
Vu B Trinh
Eswari Gudipati
Alexander Friend
Nelson A Page
Mark A Agius
David P Richman
Robert H Fairclough
|
| method: |
western blot
|
| name: |
Structural characterization of the main immunogenic region of the Torpedo acetylcholine receptor.
|
| description: |
The epitope-specific mAb 132A reacted with the fragment 1-161. Truncation analysis revealed reactivity with smaller fragments (1-161, 1-140, 1-130, 1-120, 1-110 and 1-90), but not with 1-77. Further mutational analysis of the alpha subunit N-terminus showed that the epitope consisted of two segments, aa1-12 and aa65-79. A third segment (aa110-115) was shown to play a role in the stabilization of the folding of the center MIR segment.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |