Water channel in the binding site of a high affinity anti-methotrexate antibody. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Water channel in the binding site of a high affinity anti-methotrexate antibody.

Dataset

identifier:	2110258
description:	epitope description:methotrexate host organism:Mus musculus antibody name:ADD056
aggregation:	instance of dataset
availability:	available
primaryPublications:	24832237

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1027970
landingPage:	http://www.iedb.org/assay/2110258

Publication

type:	Literature
publicationVenue:	Biochemistry
dates:	2014

Dimension

study type:	b cell assays
subject species:

Person

fullName:	Susan Gayda Kenton L Longenecker Sharmila Manoj Russell A Judge Sylvia C Saldana Qiaoqiao Ruan Kerry M Swift Sergey Y Tetin

Data Type

method:	binding assay

Study

name:	Water channel in the binding site of a high affinity anti-methotrexate antibody.
description:	The reaction kinetics of ADD056 Fab binding to the epitope-fluorescein conjugate were determined at various temperatures. A rise in temperature from 15 to 40 °C accelerates the dissociation of the complex from k off = 0.2 × 10^–4 to 6.3 × 10^–4 s^–1, a more than 30-fold increase.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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