B-cell epitopes in GroEL of Francisella tularensis. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

B-cell epitopes in GroEL of Francisella tularensis.

Dataset

identifier:	2113790
description:	epitope description:NTTIIDGAGEKEAIAKRINVIK antigen name:60 kDa chaperonin host organism:Mus musculus BALB/c antibody name:Ab64
aggregation:	instance of dataset
availability:	available
primaryPublications:	24968190

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1027962
landingPage:	http://www.iedb.org/assay/2113790

Publication

type:	Literature
publicationVenue:	PLoS One
dates:	2014

Dimension

study type:	b cell assays
subject species:	Francisella tularensis subsp. holarctica LVS

Person

fullName:	Zhaohua Lu Michael J Rynkiewicz Guillermo Madico Sheng Li Chiou-Ying Yang Hillary M Perkins Seshi R Sompuram Vani Kodela Tong Liu Timothy Morris Daphne Wang Marly I Roche Barbara A Seaton Jacqueline Sharon

Data Type

method:	hydrogen/deuterium exchange

Study

name:	B-cell epitopes in GroEL of Francisella tularensis.
description:	The epitope was deduced. The epitope of mAb Ab64 on rGroEL from strain LVS was mapped by hydrogen/deuterium exchange-mass spectroscopy (DXMS). The region of residues 329-350 showed reduced deuterium exchange in the presence of the antibody. This region included residue K344, but not residue Y476, which were found to be epitope residues by point mutation analysis. The authors suggest that mutation Y476E, which abolished Ab64 binding exerts an allosteric effect on the structure of the epitope.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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