Substitution at aspartic acid 1128 in the SARS coronavirus spike glycoprotein mediates escape from a S2 domain-targeting neutralizing monoclonal antibody.

Title:

Substitution at aspartic acid 1128 in the SARS coronavirus spike glycoprotein mediates escape from a S2 domain-targeting neutralizing monoclonal antibody.

Dataset

identifier:	2113960
description:	epitope description:VIGIINNTVYDPLQPELDSF antigen name:Spike glycoprotein host organism:Mus musculus BALB/c antibody name:1A9
aggregation:	instance of dataset
availability:	available
primaryPublications:	25019613

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1028004
landingPage:	http://www.iedb.org/assay/2113960

Publication

type:	Literature
publicationVenue:	PLoS One
dates:	2014

Dimension

study type:	b cell assays
subject species:	SARS coronavirus

Person

fullName:	Oi-Wing Ng Choong-Tat Keng Cynthia Sau-Wai Leung J S Malik Peiris Leo Lit Man Poon Yee-Joo Tan

Data Type

method:	immunoprecipitation

Study

name:	Substitution at aspartic acid 1128 in the SARS coronavirus spike glycoprotein mediates escape from a S2 domain-targeting neutralizing monoclonal antibody.
description:	The epitope specific mAb 1A9 immunoprecipitated human SARS coronavirus HKU-39849 S protein from lysates of transfected 293 FT cells. The antibody showed reduced immunoprecipitation of S protein with mutation D1128A, identified in escape mutants selected in the presence of 1A9. S protein with another escape mutation N1056K did not show reduced recognition by 1A9.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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