Non-stoichiometric O-acetylation of Shigella flexneri 2a O-specific polysaccharide: synthesis and antigenicity.

Title:

Non-stoichiometric O-acetylation of Shigella flexneri 2a O-specific polysaccharide: synthesis and antigenicity.

identifier:	2111078
description:	epitope description:alpha-L-Rhap-(1->2)-alpha-L-Rhap-(1->3)-[alpha-D-Glcp-(1->4)]-alpha-L-Rhap-(1->3)-beta-D-Glcp6,NAc2-(1->2)-alpha-L-Rhap-(1->2)-alpha-L-Rhap-(1->3)-[alpha-D-Glcp-(1->4)]-alpha-L-Rhap-(1->3)-beta-D-GlcpNAc,OCH2CH2NH2 antigen name:lipopolysaccharide host organism:Mus musculus BALB/c antibody name:F22-4, A2-1, D15-7, C1-7, E4-7
aggregation:	instance of dataset
availability:	available
primaryPublications:	24836582

study type:	b cell assays
subject species:	Shigella flexneri 2a

fullName:	Charles Gauthier Pierre Chassagne Franç ois-Xavier Theillet Catherine Guerreiro oise Thouron Farida Nato Muriel Delepierre Philippe J Sansonetti Armelle Phalipon Laurence A Mulard

method:	antigen inhibition (~ IC50)

name:	Non-stoichiometric O-acetylation of Shigella flexneri 2a O-specific polysaccharide: synthesis and antigenicity.
description:	The epitoipe inhibited the binding of the mAb F22-4 (IgG1) to Shigella flexneri 2a LPS. The epitope also inhibited the binding of SF2a-specific protective mAbs A2-1 (IgG2a; IC50 = 2 µM) , D15-7 (IgG1; IC50 = 21 µM) , C1-7 (IgG3; IC50 = 22 µM) and E4-7 (IgG2b; IC50 = 7 µM). The authors conclude that while the 6D′-acetate has no major influence on Ab recognition, the 3A-O-acetyl group displays a striking mIgG-specific effect on Ab binding. Only mAb F22-4 is able to recognize diversely O-acetylated segments of the SF2a O-Ag chain. None of the two acetates present on the SF2a O-Ag adds significantly to broad Ab recognition.

name:	iedb
homePage:	http://www.iedb.org

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