A G-quadruplex-containing RNA activates fluorescence in a GFP-like fluorophore. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

A G-quadruplex-containing RNA activates fluorescence in a GFP-like fluorophore.

Dataset

identifier:	2116307
description:	epitope description:5'-GAAACAC-3' RNA fragment host organism:Mus musculus antibody name:BL3-6
aggregation:	instance of dataset
availability:	available
primaryPublications:	24952597

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1027979
landingPage:	http://www.iedb.org/assay/2116307

Publication

type:	Literature
publicationVenue:	Nat Chem Biol
dates:	2014

Dimension

study type:	b cell assays
subject species:

Person

fullName:	Hao Huang Nikolai B Suslov Nan-Sheng Li Sandip A Shelke Molly E Evans Yelena Koldobskaya Phoebe A Rice Joseph A Piccirilli

Data Type

method:	x-ray crystallography

Study

name:	A G-quadruplex-containing RNA activates fluorescence in a GFP-like fluorophore.
description:	The crystal structure of Fab BL3-6 in complex with the RNA 84-nucleotide aptamer Spinach was determined by molecular replacement. The wild type stem-loop (UUCG) of the Spinach aptamer was replaced with a pentaloop hairpin graft (GAAACAC; residues 37-43) from the class I ligase ribozyme to create a binding site for Fab BL3-6 to be used as a crystallization chaperone.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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