Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 2119098 |
| description: |
epitope description:A: H24, H44, Q46, S304, M305, B: V364, D365, G366, W367, K384, T387, Q388, I391, T395, V398, N399, I402
antigen name:Hemagglutinin
host organism:Homo sapiens
antibody name:mAb F10
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| aggregation: |
instance of dataset
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| availability: |
available
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| primaryPublications: |
19234466 |
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1013582 |
| landingPage: |
http://www.iedb.org/assay/2119098 |
| type: |
Literature
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| publicationVenue: |
Nat Struct Mol Biol
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| dates: |
2009
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| study type: | b cell assays |
| subject species: | Influenza A virus (A/Viet Nam/1203/2004(H5N1)) |
| fullName: |
Jianhua Sui
William C Hwang
Sandra Perez
Ge Wei
Daniel Aird
Li-mei Chen
Eugenio Santelli
Boguslaw Stec
Greg Cadwell
Maryam Ali
Hongquan Wan
Akikazu Murakami
Anuradha Yammanuru
Thomas Han
Nancy J Cox
Laurie A Bankston
Ruben O Donis
Robert C Liddington
Wayne A Marasco
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| method: |
biological activity
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| name: |
Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses.
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| description: |
The residue numbering in the manuscript refers to the positions in the HA1 (H18, H38, Q40, S291, M292) and HA2 (V18, D19, G20, W21, K38, T41, Q42, I45, T49, V52, N53, I56) chains cleaved from the hemagglutinin precursor, and follows the structure of H3 in [PDB 2HMG].
The epitope specific mAb was expressed as a scFV-Fc construct or was reconstituted as a full human IgG1 from the scFV construct. It showed neutralizing activity towards the H5 subtype, clade 0 (A/HongKong/483/97), clade 1 (A/Viet Nam/1203/2004 and A/Thailand/2-SP-33/2004) and clade 2.1 (A/Indonesia/5/2005) viruses, as well as other group 1 viruses (H1N1, H2N2, H6N1, H6N2, H8N4 and H9N2 viruses, and H11-pseudotyped VLPs). It failed to neutralized group 2 (H7 and H3 subtype viruses). Analysis of the neutralizing activity with H5-pseudotyped VLPs or in cells transfected with the HA molecule showed that the nAb inhibits membrane fusion and syncitia formation rather than receptor binding.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
