How an alloreactive T-cell receptor achieves peptide and MHC specificity. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

How an alloreactive T-cell receptor achieves peptide and MHC specificity.

Dataset

identifier:	3339213
description:	epitope description:KLVALGINAV antigen name:Genome polyprotein host organism:Homo sapiens t cell receptor:HCV1406 mhc allele name:HLA-A*02:01
aggregation:	instance of dataset
availability:	available
primaryPublications:	28572406

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1031770
landingPage:	http://www.iedb.org/assay/3339213

Publication

type:	Literature
publicationVenue:	Proc Natl Acad Sci U S A
dates:	2017

Dimension

study type:	t cell assays
subject species:	Hepatitis C virus

Person

fullName:	Yuan Wang Nishant K Singh Timothy T Spear Lance M Hellman Kurt H Piepenbrink Rachel H McMahan Hugo R Rosen Craig W Vander Kooi Michael I Nishimura Brian M Baker

Data Type

method:	binding assay

Study

name:	How an alloreactive T-cell receptor achieves peptide and MHC specificity.
description:	The affinity of epitope-specific HCV1406 TCR for the epitope/A2 complex was determined by surface plasmon resonance. The affinity to the p1 Lys→Ala (K1A) NS3 variant was reduced to KD ≥170 μM. Mutation of polymorphic residues Arg65 and Lys66 in the HLA-A2 alpha chain drastically reduced binding by the HCV1406 TCR. Mutation of other polymorphic sites, Ala69, Val76, and Gly79, also reduced binding to various extents..

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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